Plasmodium-infected blood cells analyzed and sorted by flow fluorimetry with the deoxyribonucleic acid binding dye 33258 Hoechst.
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چکیده
منابع مشابه
Binding Mechanisms of Methylene Blue and Hoechst 33258 with Dna
In this work the data of methylene blue (MB) and Hoechst 33258 interaction with DNA are presented. It has been shown that at 0.002 M ionic strength of solution MB is bound to double-stranded DNA by three modes: intercalation, semi-intercalation and electrostatic. It was revealed that at higher ionic strengths of solution complete intercalation of MB becomes thermodynamically unacceptable. It wa...
متن کاملDifferent Modes of Binding of Hoechst 33258 with Dna
Spectrophotometric study of Hoechst 33258 binding to DNA has been investigated. It was shown that this ligand forms at least two types of complexes at 0.002 M and 0.02M ionic strengths of solution. One of them is strong, the other is weak. It was revealed that at 0.02M ionic strength of solution, the binding constant value by strong mode is one order higher than at 0.002M. It was obtained that ...
متن کاملAmyloglucosidase suppresses interference by glycogen in the quantification of DNA using the Hoechst 33258 dye.
The Hoechst dye fluorescence technique, which is used for the quantitative determination of DNA (12), requires high saline concentrations at neutral pH to reach maximal fluorescence of the DNA-dye complex and to render the DNA fully accessible to the dye by dissociating foreign molecules (3,12). In conventional assays, the DNA is alcohol-precipitated and then resuspended in buffer containing 2 ...
متن کاملMeasurement of cell numbers in microtiter culture plates using the fluorescent dye Hoechst 33258.
A sensitive fluorimetric assay based on DNA binding of the fluorescent dye Hoechst 33258 was developed to quantitate cell numbers in microtiter plates. The cells were fixed and incubated with the dye solution. The bound dye was extracted with ethanol and fluorescence was measured in a spectrofluorimeter. The method is sensitive for as little as a thousand cells and is particularly useful for th...
متن کاملInteraction of Hoechst 33258 and ethidium with histone1-DNA condensates.
We report structural and dynamical aspects of DNAs from various sources including synthetic oligonucleotides in bulk buffer and as a complex with histone1 (H1). High-resolution transmission electron microscopic (HRTEM) studies reveal the structural change of the DNAs upon complexation with H1 leading to formation of compact-globular and hollow-toroidal particles. In order to explore the functio...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1979
ISSN: 0022-1554,1551-5044
DOI: 10.1177/27.4.87413